Lipid-Target Assay Sandbox

Lipid Probing

Explore lipid-target staining on nanoscale vesicles using lipid mol%, vesicle geometry, probe size, Alexa Fluor 647 spacing, and assay stoichiometry. This app is intentionally lipid-only, so the controls stay focused on membrane targets instead of mixed protein and lipid scenarios.

Target count from lipid mol% Packing vs assay stoichiometry AF647 proximity and trap spacing

Browser Model

Fast Preview With Optional Monte Carlo

Monte Carlo refinement

Preview is always instant. Run Monte Carlo when you want a discrete random-packing refinement for the current settings.

Monte Carlo repeats

runs

Preview

Fast preview is current. Click Run Monte Carlo to refine the exact current settings.

Software Guide

How does this work?

Open a compact overview of how lipid mol%, vesicle geometry, binding ceilings, fluorophore proximity, and assay stoichiometry are combined.

Lipid Target

Geometry

Target lipid preset

Target distribution

Particle diameter

Target lipid fraction

mol%

Lipid area per molecule

nm^2

Headgroup footprint

Accessible EV surface

Accessible target fraction

Binding-active fraction

Vesicle View

Balanced

sampled target lipids bound probe

Packing

still bindable by geometry

AF647 Proximity

of labels within the AF647 homo-FRET range

Accessible target patch area 0 nm^2

Target close-pack limit 0

Bound-complex ceiling 0

Predicted bound probes 0

Equivalent target spacing 0 nm

Nearest fluorophore neighbor 0 nm

Parameter Sweep

Hold others fixed

Variable

Include probes

Surface Packing

AF647 Homo-FRET

AF647 Trap Radius

Interpretation Notes

Probe And AF647

Binder

Probe type

AF647 attached Include AF647 on the probe

Fluorophore

Fixed fluorophore Alexa Fluor 647

Approx. homo-FRET radius 4.0 nm

Approx. trap radius 1.8 nm

Probe footprint

Extra steric buffer

AF647 shell offset

Fluorophores per probe

count

Assay Stoichiometry

Standalone

Particle concentration

particles/mL

Probe concentration

Effective probe availability

Available probes per particle n/a

Geometry-only binding ceiling n/a

Effective final ceiling n/a

Likely main bottleneck n/a

Mol% Risk Guide

Current probe

Packing concern begins near n/a

Probe supply begins to limit near n/a

Brightness / homo-FRET concern begins near n/a

Coverage And Loss

Outcome

Target surface coverage 0%

Surface coverage by bound probe 0%

Binding retention 0%

Labels within AF647 homo-FRET range 0%